Gel Electrophoresis Buffers and Diluents
Medchemexpress LLC Sodium Citrate Buffer, 0.5M, pH 5.0 | 6132-04-3 | MFCD00150031 | 100 ML
Sodium Citrate Buffer, 0.5M, pH 5.0 is a commonly used buffer composed of citric acid and sodium citrate, widely utilized in molecular biology, immunohistochemistry (IHC), and biochemistry. It serves various critical functions in laboratory settings.
- Used in heat-induced epitope retrieval (HIER) to reverse antigenicity loss in formalin-fixed paraffin-embedded tissues.
- Can be applied as an RT-PCR enhancement reagent.
- Utilized as a vehicle for Captisol in animal tumor model studies.
Bioworld SpotQC hybridization Buffer, 20 mL
SpotQC Hybridization Buffer is an optimized hybridization solution specifically formulated for use with IDT’s SpotQC detector oligos. It also allows for the dilution of the SpotQC detector oligo up to 20-fold while maintaining the superior detection of a broad range of oligo-probe lengths and GC compositions.
Bioworld Protein-Coupling Buffer Kit, 1 Kit
bioWORLD's Protein Coupling Buffer kit contains reagents/buffers necessary for convenient protein coupling to CNBr activated Sepharose or Separopore.Kit Components:Swell Buffer: 1L Coupling Buffer: 1L Wash buffer: 1LBlocking Buffer: 50mlEquilibrating Buffer: 100 mlReagents provided are sufficient for 25ml of gel.
Revvity Health Sciences Inc HTRF Human Phospho-Aurora A (Thr288) Detection Kit, 10,000 Assay Points
HTRF Human Phospho-Aurora A (Thr288) Detection Kit, 10,000 Assay Points
Medchemexpress LLC Lipopolysaccharide-binding protein (human, HEK293, His) | 100UG
Recombinant human lipopolysaccharide-binding protein produced in HEK293 cells with a C-terminal His tag. LBP binds the lipid A moiety of bacterial LPS and facilitates CD14 interaction, making it suitable for studies of innate immune signaling, LPS-binding assays, and use as a positive control in immunoassays.
- Mammalian expression in HEK293 for native glycosylation.
- C-terminal His tag for purification and detection.
- Full-length sequence corresponds to residues A26-V481 (456 aa).
- Apparent molecular weight ~55-75 kDa due to glycosylation.
- Low endotoxin level (<1 EU/μg) suitable for cell-based assays.
- Datasheet guidance: avoid reconstituting below 100 μg/mL.
Zymo Research Corporation Sequencing Wash Buffer, 70 ml
Sequencing Wash Buffer (70 ml). For use in the ZR DNA Sequencing Clean-up Kit. Sold individually or as a part of the ZR DNA Sequencing Clean-up product line from Zymo Research.
Ethos Biosciences Dilurex HepCas+ Buffer, 100ml, Ethos
Dilurex HepCas+ Buffer is a HEPES buffer system optimized for a biocompatible pH range of 7.2-7.4 with an isotonic (0.154M) ionic stregth keeping proteins stable. It contains Casein as a carrier protein that is specially formulated to reduce non-specific binding and protease activity. Try this if you have non-specific binding using an Albumin based buffer system. Match with Casein blocking buffer for optimal performance. Formulated to optimize ELISA assays with decades of stabiilty.
Bioworld Citrate Buffer, 0.5M, pH 8.0, 250 mL
The citrate buffer is composed of Citric acid monohydrate (C6H8O7 • H2O, MW 210.14) and Trisodium citrate dehydrate (C6H5O7Na3 • 2H2O, MW 294.12) mixed in a fixed ratio to attain the desired pH. As a conjugate base of a weak acid, citrate can perform as a buffering agent or acidity regulator, resisting changes in pH. May be used for antigen retrieval of tissue samples. The citrate solution is designed to break protein cross-links; thus, unmasking antigens and epitopes in formalin-fixed and paraffin embedded tissue sections, resulting in enhancing staining intensity of antibodies. Citrate has anticoagulant activity as a calcium chelator.Technical Information :citrate buffer (pK1): 3.13 (25°C, pH range: 2.2-6.5)citrate buffer (pK2): 4.76 (25°C, pH range: 3.0-6.2)citrate buffer (pK3): 6.4 (25°C, pH range: 5.5-7.2)Warning:For laboratory use only.Not fit for agricultural, household, clinical use or humanconsumption.PH Value: 8.0
![Alkali Scientific ACES [N (2 Acetamido) 2 aminoethane], 1 Kg](https://assets.fishersci.com/TFS-Assets/CCG/product-images/5000660794.jpg-250.jpg)
Alkali Scientific ACES [N (2 Acetamido) 2 aminoethane], 1 Kg
ACES (N-(2-Acetamido)-2-aminoethane) is a high-purity biological buffer available in powder form. This reagent is commonly used in various biological and biochemical applications, including enzyme activity assays and protein analysis. ACES provides a stable pH environment, particularly in reactions where a low-pH buffer is required. The 1 Kg quantity of ACES is ideal for laboratories with high throughput, enabling consistent results in experimental protocols that require precision buffering. ACES buffers are especially effective in maintaining pH stability in protein studies, DNA/RNA analysis, and microbial growth experiments. This product is commonly used in life sciences research, particularly in proteomics and molecular biology workflows.
ILEX LIFE SCIENCES LLC 3D LIFE 10X CBBUFF PH5.5 600UL
3-D Life 10x CB-Buffer (pH 5.5), buffer component for fast-gelling 3-D Life Hydrogels (600 ul). INTRO: 3-D Life technology, manufactured in Germany by Cellendes GmbH, is an integrated system for the generation of biomimetic hydrogels used in 3D cell culture. 3-D Life represents the most flexible system for biomimetic hydrogel design. DESCRIPTION: 3-D Life 10x CB-Buffer (pH 5.5) is a 10x concentrated salt and buffer solution which is diluted to physiological strength when used in the 3-D Life system. It is used for the formation of fast gelling hydrogels where it provides a cell environment at pH 5.5, which is compatible with most cell types. In conjunction with 3-D Life 10x CB-Buffer (pH 7.2) it is used to generate pHs of intermediate values to modify the gelation kinetics of fast gelling gels. APPLICATION: Used with other components of the 3-D Life Hydrogel System to establish 3D cell cultures. QUANTITY: 600 µl. Sufficient for at least 6 ml 3-D Life Hydrogel.
Electron Microscopy Sciences Earl's Balanced Salts Formula I 1 LT
All of our Earle's Balanced Salt Solution contains: Potassium Chloride, Sodium Chloride, Sodium Phosphate Monobasic, and D-glucose. Some formulations may contain additional components. Use the table below as a quick reference for identifying the one that best suits your application. All of the solutions are prepared 1X strength.